Journal: Biomedical Reports

Article Title: Association between miRNA expression profiles and polymorphisms of dihydropyrimidine dehydrogenase drug-metabolizing gene in patients with colorectal cancer receiving 5-fluorouracil

doi: 10.3892/br.2025.2053

Figure Lengend Snippet: miR expression profiles in patients with VT ( DPYD 85T>C) and WT colorectal cancer. A total of 43 miRs were detected in the plasma of patients with VT (n=4) and WT colorectal cancer (n=5) using miR array. (A) Heat map of differential expression of miRs in VT compared with WT colorectal cancer. Red and green show up- and downregulation, respectively. (B) Scatter plot showing miR profiles between VT and WT colorectal cancer. Each dot represents the fold-change in expression of miRNA. Red, green, and black dots represent up- and downregulated and unchanged miRNAs, respectively. miR, microRNA; VT, Variant; DPYD , dihydropyrimidine dehydrogenase gene; WT, Wild type; min, minimum; avg, average; max, maximum; sn, small nuclear.

Article Snippet: The miRNA expression profiles were analyzed using RT-PCR with a customized miRNA array panel (cat. no. 217184; Qiagen GmbH) coated with specific primers for 43 miRNAs (sequences not provided) involved in the 5-FU metabolic pathway. miR target sequences are listed in .

Techniques: Expressing, Clinical Proteomics, Quantitative Proteomics, Variant Assay

Journal: Biomedical Reports

Article Title: Association between miRNA expression profiles and polymorphisms of dihydropyrimidine dehydrogenase drug-metabolizing gene in patients with colorectal cancer receiving 5-fluorouracil

doi: 10.3892/br.2025.2053

Figure Lengend Snippet: miRNA expression profiles in patients with VT ( DPYD 1896T>C) and WT colorectal cancer. A total of 43 miRs were detected in the plasma of patients with (n=3) and WT colorectal cancer (n=3). (A) Heat map of differential expression of miRs in VT compared with WT colorectal cancer. Red and green show up- and downregulation, respectively. (B) Scatter plot showing miR profiles between VT and WT colorectal cancer. Each dot represents the fold-change in expression of miRNA. Red, green, and black dots represent up- and downregulated and unchanged miRNAs, respectively. miR, microRNA; VT, Variant; DPYD , dihydropyrimidine dehydrogenase gene; WT, Wild type; min, minimum; avg, average; max, maximum; snRNA, small nuclear RNA.

Article Snippet: The miRNA expression profiles were analyzed using RT-PCR with a customized miRNA array panel (cat. no. 217184; Qiagen GmbH) coated with specific primers for 43 miRNAs (sequences not provided) involved in the 5-FU metabolic pathway. miR target sequences are listed in .

Techniques: Expressing, Clinical Proteomics, Quantitative Proteomics, Variant Assay

Journal: MedComm

Article Title: Dormant Metastases Exhibit a Unique Phenotype Primarily Promoted by the Ch25h Gene and Are Maintained in Dormancy by T Lymphocytes

doi: 10.1002/mco2.70437

Figure Lengend Snippet: Genes differentially expressed in the dormant metastasis group. Heat maps are presented to illustrate the differential expression data between the three metastatic groups obtained from RNA‐Seq (A), or RT‐qPCR in genes involved in the cholesterol synthesis pathway (B), in chemokine genes (C), and in surface marker genes (D). Only genes that displayed a greater than twofold difference in expression (log2>1) with a p ‐value less than 0.001 were identified and plotted in the heatmap. The expression values are presented as log2 values and are scaled by gene. In RT‐qPCR assays, the expression levels of the genes of interest were determined with respect to the levels of the β‐actin and GAPDH housekeeping genes. The data for the overt‐met group were set to 1. The values represent the means of three independent experiments performed in duplicate. The statistical analysis was performed using ANOVA, followed by Tukey's post hoc test.

Article Snippet: The miProfileMouse miRNome miRNA qPCR Array (catalog QM002; GeneCopoeia) was used to analyze 834 mouse miRNAs.

Techniques: Quantitative Proteomics, RNA Sequencing, Quantitative RT-PCR, Marker, Expressing

Journal: MedComm

Article Title: Dormant Metastases Exhibit a Unique Phenotype Primarily Promoted by the Ch25h Gene and Are Maintained in Dormancy by T Lymphocytes

doi: 10.1002/mco2.70437

Figure Lengend Snippet: MicroRNAs that are differentially expressed in the dormant metastasis group. (A) Heat map is presented to illustrate the differential expression data between the three metastatic groups. (B) miRNA enrichment analysis of miRNAs differentially expressed in dormant‐met group (miEAA). The expression of 834 miRNAs was measured using the ‘mouse miRNome qPCR arrays 18.0’ (Genecopoeia). Only miRNAs that displayed a greater than twofold difference ( p < 0.01) when comparing the dormant‐met group with the nude‐met and overt‐met groups were plotted. The expression levels of the miRNAs of interest were determined with respect to the levels of six housekeeping snRNAs (MK1‐MK6). The data for the overt‐met group were set to 1. The values are presented as the means of three independent experiments, each performed in duplicate. The statistical analysis was conducted using an ANOVA test, followed by a Tukey's post hoc test.

Article Snippet: The miProfileMouse miRNome miRNA qPCR Array (catalog QM002; GeneCopoeia) was used to analyze 834 mouse miRNAs.

Techniques: Quantitative Proteomics, Expressing

Journal: bioRxiv

Article Title: Perfusion Bioreactor Culture Incorporating Mechanical Confinement Enhances Mesenchymal Stem Cell Extracellular Vesicle Production and Wound Healing Potential

doi: 10.1101/2025.08.12.669872

Figure Lengend Snippet: A) Timeline of experiment setup and injection schedule. Mice received injections of 1.1×10 10 EVs or an equivalent volume of PBS on days 0, 3, 6, and 9. B) Representative photos of splinted wounds receiving each treatment over 14 days (n=4-6). C) Percent wound closure based on wound area over 14 days (n=4-6). D) Fold change of M1 markers CD86, iNOS, and TNF11 and E) M2 markers IL-10, CD206, and Arg1 in harvested tissue from mice receiving flask and bioreactor EV injections relative to tissue from mice receiving PBS, as quantified by qPCR. Data represents 4 biological replicates analyzed with 3 technical replicates each per treatment group (n=4). F) Length of regenerating epithelium from H&E staining as a percentage of the length of the wound bed (n=4-6). G) Collagen deposition from Masson’s Trichrome staining quantified as a percentage of the total tissue volume (n=4-6). G) Granulation tissue area from H&E staining (n=4-6). I) CD31 fluorescence intensity normalized to DAPI over multiple fields of view and representative confocal microscopy images taken at 10X magnification (n=4-5). All values expressed as mean +/- standard error of the mean (ns – no significance; * p<0.05; ** p<0.01).

Article Snippet: Resulting cDNA was analyzed in a human MSC exosome miRNA qPCR array (GeneCopoeia; QM0460B6) per the manufacturer’s protocol.

Techniques: Injection, Staining, Fluorescence, Confocal Microscopy

Journal: bioRxiv

Article Title: Perfusion Bioreactor Culture Incorporating Mechanical Confinement Enhances Mesenchymal Stem Cell Extracellular Vesicle Production and Wound Healing Potential

doi: 10.1101/2025.08.12.669872

Figure Lengend Snippet: A) Heat map of the miRNA content of 5 µm static and 5 µm bioreactor EVs represented as the log2(fold change) relative to flask EVs (n≥2). B) Volcano plot comparing the miRNA within static and flask EVs, with the horizontal line at 1.3 (p = 0.05) representing statistical significance (n≥2). C) Volcano plot comparing the miRNA within bioreactor and flask EVs, with the horizontal line at 1.3 (p = 0.05) representing statistical significance. The labeled points indicate those in common between both static and bioreactor EVs (n≥2). All data represents the average of at least 2 independent experiments.

Article Snippet: Resulting cDNA was analyzed in a human MSC exosome miRNA qPCR array (GeneCopoeia; QM0460B6) per the manufacturer’s protocol.

Techniques: Labeling